NEXome XP Panel v1.0

• Clinvar variants in non-coding region
NEXome XP Panel v1.0 provides expanded coverage of clinically relevant variants in non-coding genomic regions.。

• Expanded coding region coverage
NEXome XP Panel v1.0 further includes the GENCODE annotation of protein-coding regions, and provides a comprehensive coverage of IG and TR coding genes.
• SNP skeleton of whole genome
Based on the 1000 Genomes database, we mainly select >9,000 loci with high MAF values and high heterozygosity in the Chinese population, covering the entire human genome at approximately 300 Kb intervals. It provides richer and more evenly distributed information on the genomic composition based on the whole exome, and can be used for detection of CNVs and loss of heterozygosity.

• Common gene fusion in solid tumors

Contains intron regions and non-coding regions related to common fusions and can be used to analyze fusion loci in solid tumors.

ALK intron 18 - 19	BCL2 3'UTR	BCR intron 8, 13 - 14	BRAF intron 7 - 10	BRCA1 intron 2, 7 - 8, 12, 16, 19 - 20	BRCA2 intron 2	CD74 intron 6 - 8	EGFR intron 7, 15, 24 - 27	ETV4 intron 5 - 6	ETV5 intron 6 - 7	ETV6 intron 5 - 6
EWSR1 intron 6 - 13	EZR intron 9 - 12	FGFR1 intron 1, 5, 17	FGFR2 intron 1, 17	FGFR3 intron 17	FLI1 intron 3 - 8	KIT intron 16	KMT2A intron 6 - 11	MET intron 1, 14	MSH2 intron 5	MYB intron 14
MYC intron 1	NOTCH2 intron 26	NTRK1 intron 8 - 10	NTRK2 intron 12, 15	NTRK3 intron 13 - 14	NUTM1 intron 1	PDGFB intron 1	PDGFRA intron 7, 9, 11	RAF1 intron 4 - 9	RARA intron 2	RET intron 7 - 11
ROS1 intron 31 - 35	RSPO2 Upstream, 5'UTR, exon 1 - 2, intron 1		SDC4 intron 2	SLC34A2 intron 4	TMPRSS2 intron 1 - 3

• Classical microsatellite loci
The NEXome Core Panel itself covers a large number of microsatellite loci, and the addition of 15 optimally designed MSI classical loci facilitates the comparison between different microsatellite instability analytical methods as well as the optimization and calibration of the analytical process.


BAT-25

BAT-26

BAT-40

BAT-RII

NR-21

NR-22

NR-24

NR-27

MONO-27

D2S123

D5S346

D17S261

D17S520

D17S250

D18S34

Capture Performance

Fig 1. Capture performance of NEXome XP Panel v1.0 in different gDNA libraries.  The libraries were prepared using NadPrep DNA Universal Library Preparation Module Series coupled with NadPrep Universal Stubby Adapter (UDI) Module and NadPrep Universal Adapter (MDI) Module (for MGI) from different gDNA. NEXome XP Panel v1.0 were respectively used to complete hybridization capture. The BWA was used for alignment of raw reads to the reference genome (hg38). A. Mappability and On-target rate; B. Targeted covered; C. Coverage uniformity and consistency; D. The unbiased of GC coverage.
Note: The gDNA_1-4 samples were: Human Male Genomic DNA (Promega-male, G1471); Human Female Genomic DNA (Promega-female, G1521); PancancerLight 800 gDNA Reference Standard with 100 ng (Genewell, GW-OGTM800); Cell Line gDNA Standard (Coriell, NA12878), respectively. Sequencing platform: Illumina Novaseq 6000，PE150；DNBSEQ-G400，PE150.

Variant Analysis

Fig 2. Consistency of the allele frequencies in NEXome XP Panel v1.0 capture data with the nominal frequencies of the standards.The libraries were prepared using NadPrep DNA Universal Library Preparation Kit Series and NEXome XP Panel v1.0 were respectively used to complete hybridization capture. VarDict was used for variant calling. Sequencing platform: Illumina Novaseq 6000，PE150；DNBSEQ-G400，PE150.
Note: Sample type: PancancerLight 800 gDNA Reference Standard with 100 ng (Genewell, GW-OGTM800).

High Confidence Variant Calls

Fig 3. Sensitivity and positive predictive value (PPV) of NEXome XP Panel v1.0 for NA12878 mutation identification.A.SNPs；B. Indels。

Note: Sample type: Cell Line gDNA Standard (Coriell, NA12878). Sequencing platform: Illumina Novaseq 6000，PE150；DNBSEQ-G400，PE150.

For research use only. Not for use in diagnostic procedures.