What are possible causes of no amplification product or low amplification yield when using the NadPrep Single Cell WGA Kit? How to solve them?

Cell loss during isolation: if cells are lost during isolation or transfer, inspect and optimize the cell isolation and collection workflow. Recollect samples and confirm that each reaction tube contains a cell before proceeding.

Insufficient purity of gDNA: residual wash buffers, ethanol or other contaminants remaining after purification can inhibit the amplification reaction. Ensure ethanol is fully evaporated after purification, resuspend/dilute purified gDNA in Nuclease Free Water, and verify accurate quantification of the input samples.