Although microgram-level amplified DNA was obtained using the NadPrep Single Cell WGA Kit, what may cause abnormal allelic loss or chromosomal abnormalities, and how to solve them?

I. If the initial input samples are cells: apoptosis of cells prior to lysis can lead to DNA degradation, which may produce allelic loss or chromosomal abnormalities. It is recommended to assess cell viability and proceed with lysis and amplification immediately when the cell status is acceptable. If immediate amplification is not possible, store lysed cells at -25 ~ -15℃ for ≤ 7 days to preserve genomic integrity.

II. If the initial input samples are bulk DNA: partial degradation of the input gDNA can likewise cause such artifacts. Use intact, non-degraded gDNA, or increase the initial input amount as appropriate.