What are the possible reasons for relatively high library yield after hybrid capture with μCaler? How to solve it?

Possible Reason

Solution

Relatively high starting input

Quantification by Qubit™ 3.0 is recommended.

Relatively high amplification cycles

Explore the optimal amplification cycles according to the recommended protocol in the operating guideline.

The elution is not performed according to operational guideline, leading to serious off-target.

The elution is performed in strict accordance with the requirements of operating guideline.