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What can cause low library yield when using the NadPrep EZ RNA Library Preparation Kit? How to solve it?

View: 49 / Time: 2025-11-04
  • RNase contamination: Use RNase-free consumables throughout the workflow and perform all the operations in RNase-free conditions. RNase contamination will reduce reverse transcription efficiency and low library yield.
  • Low sample purity: Residual contaminants in RNA (e.g. chelators, guanidine salts, phenol, ethanol) may inhibit the activity of reverse transcriptase. It is recommended to purify RNA according to Step 7: Post-amplification Cleanup in the use manual using 2X NadPrep SP Beads and elute in Nuclease Free Water.
  • Poor sample quality: For low-quality RNA samples, follow the user manual’s recommendations to increase the no. of PCR cycles appropriately.
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