How to deal with the issue of excessively large or double peaks in RNA & DNA Co-prepared library fragments?

If the nucleic acid samples contain high concentrations of EDTA or have an excessively high pH, it can affect the efficiency of DNA digestion. It is recommended to purify nucleic acid samples using 2X NadPrep SP Beads, replacing the elution reagent with Nuclease Free Water before proceeding with library preparation.