How to improve the detection rate of low-frequency mutational signals with NGS protocols?

① Increase the sample input:The more sample input , the copies of support low-frequency mutation detection will be more；

② Increase the number of tracked mutations: the higher the number of mutation sites, the probability of detection will be higher.

Note：a. The more sample input, the copy number that supports low-frequency mutation detection will be more higher b. For the same sample size, the higher the number of mutation sites, the higher the detection probability;