What are the possible reasons for Low library yield after hybrid capture with μCaler? How to solve it?

Possible Reason

Solution

Inaccurate starting input

In case of lower actual starting input, precise quantification by qPCR is recommended.

Reaction mixture is not completely transferred out when pipetting.

Transfer all reaction mixture from each step to a new tube when pipetting.

Some magnetic beads are discarded during hybridization and bead purification

Avoid too intense operation during the elution, and discard supernatant without losing any magnetic beads.