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Ext-RNA Control Panel

Catalog Number:

#1001652 (16 rxn)
#1001651 (96 rxn)

The ERCC RNA Spike-in Control Mixes is developed by National Institute of Standards and Technology (NIST) which is composed of a serious of RNA sequences with determined concentrations. It can derive standard curves relating read counts to RNA concentration and is widely used in performance assessment of gene expression experiments.

Ext-RNA Control Panel v1.0 targeted 24 of 92 ERCC transcripts 273-2,022 bp in length, with concentration ranging from 0.014305 attomol/μL to 937.5 attomol/μL. The 24 ERCC RNAs will serve as external controls for RNA expression quantification.

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List of Targeted 24 ERCC RNAs

Table 1. Information of targeted 24 ERCC RNAs.

ERCC ID	Concentration (attomol/μL）	Length (bp)	ERCC ID	Concentration (attomol/μL)	Length (bp)	ERCC ID	Concentration (attomol/μL)	Length (bp)
ERCC-00057	0.014305	1,021	ERCC-00058	1.831055	1,136	ERCC-00170	14.64844	525
ERCC-00017	0.114441	1,136	ERCC-00077	3.662109	743	ERCC-00084	29.29688	994
ERCC-00016	0.228882	844	ERCC-00150	3.662109	273	ERCC-00025	58.59375	1,994
ERCC-00156	0.457764	494	ERCC-00034	7.324219	844	ERCC-00071	58.59375	642
ERCC-00158	0.457764	1,027	ERCC-00085	7.324219	1,019	ERCC-00112	117.1875	1,136
ERCC-00109	0.915527	536	ERCC-00157	7.324219	1,019	ERCC-00092	234.375	1,124
ERCC-00137	0.915527	537	ERCC-00059	14.64844	1,023	ERCC-00042	468.75	1,023
ERCC-00033	1.831055	2,022	ERCC-00126	14.64844	1,118	ERCC-00108	937.5	1,022

Workflow

Fig 1. The workflow of RNA targeted capture sequencing.

Performance

ERCC Standard Validation

Fig 2. ERCC standard validation. ERCC transcripts were added to the RNA sample during the construction of RNA sequencing (RNA-seq) library or targeted capture RNA sequencing (RNACap) library. The RPKM value of ERCC spike-in correlates linearly with ERCC spike-in concentration; transcripts of targeted genes enriched in RNACap were compared to RNA-seq.

Coverage of ERCC Transcripts

Fig 3. Influence of three RNA library construction methods on coverage of ERCC transcripts. Different methods achieving the same level of coverage with RNACap presents no coverage bias. For RNACap (blue), RNA library was constructed from total RNA for targeted capture sequencing; for rRNA-depleted (orange), RNA library was constructed for direct sequencing after ribosomal RNA (rRNA) been depleted from total RNA; for total RNA (dark blue), RNA library was constructed from total RNA for direct sequencing.

Performance Repeatability

Fig 4. Performance repeatability of Ext-RNA Control Panel v1.0. The panel was spiked-in to Onco Plus Research Panel v2.0 and OnCT Research Panel v1.0 separately for targeted capture sequencing. Correlation analysis was introduced to analyze the repeatability of the log value of the RPKMs.

For research use only. Not for use in diagnostic procedures.

Catalog#	Color Of Tube Cap	Item	Volume	Package/Storage
1001652		Ext-RNA Control Panel v1.0, 16 rxn	70 μl×1	–20℃
1001651		Ext-RNA Control Panel v1.0, 96 rxn	415 μl×1	–20℃