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NanoRV Panel-Tech Access

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#1001121 (96 rxn)

NanoRV Panel v1.0-Tech Access targets human respiratory viruses including coronavirus, influenza virus, parainfluenza virus, respiratory syncytial virus, metapneumovirus, enterovirus, rhinovirus, adenovirus, and bocavirus. Notably, its up-to-date (2020 Mar) design allows for full-genome capture of the SARS-CoV-2 (2019-nCoV). This panel consists of over 16,000 biotinylated 120 nt DNA oligos and can be utilized for hybridization capture-based viral sequence enrichment.

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NanoRV Panel v1.0-Tech Access targets 36 species respiratory virus from 12 genera in 7 families. Both the reference genomic sequences and “neighbor” viral genomes have been taken into consideration in the panel design.

Table 1. Human respiratory virus targeted by NanoRV Panel v1.0-Tech Access

Family	Genus	Species covered
Coronaviridae	Alphacoronavirus ，Betacoronavirus	2 alpha and 13 beta
Orthomyxoviridae	Alphainfluenzavirus, Betainfluenzavirus, Gammainfluenzavirus	Influenza A virus, Influenza B virus, and Influenza C virus
Paramyxoviridae	Respirovirus，Rubulavirus	Human parainfluenza virus 1, 2, 3, 4
Pneumoviridae	Metapneumovirus, Orthopneumovirus	Human metapneumovirus and Human orthopneumovirus
Picornavirus	Enterovirus	Enterovirus A, B ,C ,D and Rhinovirus A, B, C
Adenoviridae	Mastadenovirus	Human adenovirus B, C, E
Parvoviridae	Bocaparvovirus	Primate bocaparvovirus 1 and Primate bocaparvovirus 2

Table 2. Refseqs with whole genome covered by NanoRV Panel v1.0-Tech Access

Performance

Fig 1. Enrichment efficiency of NanoRV Panel v1.0-Tech Access. The library was constructed from COVID-19 RNA reference (Genewell, GW-CRPM002) using NadPrep DNA Universal Library Preparation Kit (for Illumina®️) combined with NadPrep Total RNA-to-DNA Module. Custom probes targeting 7 human housekeeping genes were spiked into the NanoRV Panel v1.0-Tech Access, followed by either single or double capture sequencing. Sequencing data were analyzed with Salmon v1.2.0. Reads mapped to SARS-CoV-2 or targeted housekeeping genes were counted as on-target, while rRNAs and other transcripts as off-target.

Fig 2. The abundance of SARS-CoV-2 compared to selected housekeeping genes. The libraries constructed from COVID-19 RNA reference (Genewell, GW-CRPM002) were enriched using the NanoRV Panel v1.0-Tech Access spiked with custom probes targeting 7 human housekeeping genes. Salmon v1.2.0 was used to calculate TPM.

Fig 3. Coverage uniformity across the SARS-CoV-2 genome. The library was constructed from COVID-19 RNA reference (Genewell, GW- CRPM002) followed by targeted enrichment of SARS-CoV-2 with the NanoRV Panel v1.0-Tech Access.