Why is the dsDNA yield slightly higher when prepared using the NadPrep rRNA Fast Blocking Comprehensive Solution?

A: This solution contains a large amount of rRNA ssDNA blocking reagent. When used in conjunction with the NadPrep Total RNA-To-DNA Module, there may be a slight residual of ssDNA in the purified dsDNA product. When quantifying dsDNA using a nucleic acid fluorescence quantifier, this residual ssDNA can influence the results, leading to a slightly higher yield. However, the remaining rRNA ssDNA blocking reagent does not affect subsequent experiments. We recommend appropriate adapter dilution factors and PCR amplification cycles for different different types of RNA and different input amounts