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How to detect 0.005% mutation ?

View: 2373 / Time: 2022-11-03
A：① Increase the sample input
The lower limit of mutation detection corresponding to different experimental conditions

Library conversion rate

Sample Input

1 ng

10 ng

20 ng

30 ng

50 ng

100 ng

200 ng

300 ng

500 ng

1000 ng

20%

5.000%

0.500%

0.250%

0.168%

0.100%

0.050%

0.025%

0.017%

0.010%

0.005%

30%

3.400%

0.340%

0.170%

0.113%

0.068%

0.034%

0.017%

0.011%

0.007%

0.003%

50%

2.000%

0.200%

0.100%

0.067%

0.040%

0.020%

0.010%

0.007%

0.004%

0.002%

100%

1.000%

0.100%

0.050%

0.033%

0.020%

0.010%

0.005%

0.003%

0.002%

0.001%

When the library conversion rate is 20%, 0.005% of the mutations can be detected with a sample input of 1000 ng;
When the library conversion rate is 50%, 0.005% of the mutations can be detected with a sample input of 400 ng;
②Increase the number of mutation sites
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